Bacterial Dye obtained from pseudomonas Fluorescens for Dyeing of Cotton

Bhargava, Deepti and Jahan, Shahnaz

The world is shifting from synthetic to nature based products in today’s era. Bacterial pigment production is now one of the emerging fields of research to demonstrate its potential for various industrial applications. Therefore, present research work was carried out to optimize the growth conditions of Pseudomonas fluorescens (Pf-6), dyeing variables, to see the effect of natural mordants with dye using different mordanting methods on cotton fabric, testing of color fastness and antimicrobial activity of the dye. Maximum pigment was extracted from Pseudomonas fluorescens when it was inoculated on King’s B agar at 7pH and incubated for two to seven days at 250C in BOD incubator. Best colour was obtained on fabric when it was dyed with the dye keeping the pH 5 of the dye solution, 2 percent concentration of the dye, 1:30 material to liquor ratio and dyeing was done at 800C for 90 minutes. Overall fastness of the dyed fabric increased with mordanting.

Keywords: Textile | bacteria | pigment | modanting


Natural dyes are mainly obtained from plant sources. They have many advantages but also have certain limitations. These limitations includes low or non availability throughout the year, requirement of large amount of cultivated land for the production of plant sources, problem in transportation, lack of the reproducibility of the same shades and unstable to light and pH etc. Therefore, great concern is necessary for searching of those natural sources of textile dyes which can overcome these limitations of natural dyes. Recently, there has been increasing interest in using microorganism as a renewable source of natural dyes for textiles. Microorganisms take less time to multiply whereas plants take many days to years to grow. Moreover most of plant give dye during specific season. Thus, microorganisms can be one of the best alternate for the production of natural dyes.
Therefore, efforts have been made in the present research work to identify new bacterial source of natural dye with objectives to optimize the growth conditions of Pseudomonas fluorescens (Pf-6), to optimize the dyeing variables, to assess the color fastness of dyed fabric and to test the antimicrobial activity of dye source.

Download PDF